production on a population level and suggests AGD can

provide some information about a man

’

s reproductive

potential.

Conflicts of interest

The authors have no conflicts of interest to

disclose.

References

1. Greenham LW, Greenham V. Sexing mouse pups. Lab Anim.

1977;11:181

–

2. Hsieh MH, Breyer BN, Eisenberg ML, Baskin LS. Associations

among hypospadias, cryptorchidism, anogenital distance, and endo-

crine disruption. Curr Urol Rep. 2008;9:137

–

42.

3. Marois G. [Action of progesterone, testosterone and estradiol on the

anogenital distance and somatic sexual differentiation in rats]. Biol

Med (Paris) 1968;57:44

–

90.

4. Thankamony A, Ong KK, Dunger DB, Acerini CL, Hughes IA.

Anogenital distance from birth to 2 years: a population study.

Environ Health Perspect. 2009;117:1786

–

90.

5. Torres-Sanchez L, Zepeda M, Cebrian ME, Belkind-Gerson J,

Garcia-Hernandez RM, Belkind-Valdovinos U, et al.

Dichlorodiphenyldichloroethylene exposure during the first trimester

of pregnancy alters the anal position in male infants. Ann N YAcad

Sci. 2008;1140:155

–

62.

6. Salazar-Martinez E, Romano-Riquer P, Yanez-Marquez E,

Longnecker MP, Hernandez-Avila M. Anogenital distance in human

male and female newborns: a descriptive, cross-sectional study.

Environ Health. 2004;3:8.

7. Sathyanarayana S, Beard L, Zhou C, Grady R. Measurement and

correlates of ano-genital distance in healthy, newborn infants. Int J

Androl. 2010;33:317

–

23.

8. Eisenberg ML, Hsieh MH, Walters RC, Krasnow R, Lipshultz LI.

The relationship between anogenital distance, fatherhood, and fertil-

ity in adult men. PLoS One. 2011;6:e18973.

9. Mendiola J, Stahlhut RW, Jorgensen N, Liu F, Swan SH. Shorter

anogenital distance predicts poorer semen quality in young Men in

rochester, New york. Environ Health Perspect. 2011;119:958

–

63.

10. Eisenberg ML, Jensen TK, Walters RC, Skakkebaek NE, Lipshultz

LI. The relationship between anogenital distance and reproductive

hormone levels in adult men. J Urol. 2012;187:594

–

11. Eisenberg ML, Hsieh TC, Lipshultz LI. The relationship between

anogenital distance and age. Andrology. 2013;1:90

–

12. Eisenberg ML, Shy M, Chanc Walters R, Lipshultz LI. The relation-

ship between anogenital distance and azoospermia in adult men. Int J

Androl. 2012;35:726

–

30.

13. Eisenberg ML, Shy M, Herder D, Walters RC, Lipshultz LI. The

relationship between anogenital distance and the efficacy of varico-

cele repair. BJU Int. 2012;110:E927

–

30.

14. Castano-Vinyals G, Carrasco E, Lorente JA, Sabate Y, Cirac-

Claveras J, Pollan M, et al. Anogenital distance and the risk of

prostate cancer. BJU Int. 2012;110:E707

–

10.

15. Dean A, Sharpe RM. Clinical review: Anogenital distance or digit

length ratio as measures of fetal androgen exposure: relationship to

male reproductive development and its disorders. The Journal of

Clinical Endocrinology and Metabolism. 2013;98:2230

–

16. Behre HM, Nashan D, Nieschlag E. Objective measurement of

testicular volume by ultrasonography: evaluation of the technique

and comparison with orchidometer estimates. Int J Androl. 1989;12:

395

–

403.

17. Cooper TG, Noonan E, von Eckardstein S, Auger J, Baker HW,

Behre HM, et al. World Health Organization reference values for

human semen characteristics. Hum Reprod Update. 2010;16:231

–

45.

18. DeLong ER, DeLong DM, Clarke-Pearson DL. Comparing the areas

under two or more correlated receiver operating characteristic curves:

a nonparametric approach. Biometrics. 1988;44:837

–

45.

19. Vittinghoff E, Glidden DV, Shiboski SC, McCulloch CE. Regression

Methods in Biostatistics: Linear, Logistic, Survival, and Repeated

Measures Models. New York: Springer; 2005.

20. Larson WJ. Human Embryology. 2nd ed. New York: Churchill

Livingstone; 1997.

21. Callegari C, Everett S, Ross M, Brasel JA. Anogenital ratio: measure

of fetal virilization in premature and full-term newborn infants. J

Pediatr. 1987;111:240

–

22. Macleod DJ, Sharpe RM, Welsh M, Fisken M, Scott HM, Hutchison

GR, et al. Androgen action in the masculinization programming

window and development of male reproductive organs. Int J

Androl. 2010;33:279

–

87.

23. Welsh M, MacLeod DJ, Walker M, Smith LB, Sharpe RM. Critical

androgen-sensitive periods of rat penis and clitoris development. Int J

Androl. 2010;33:e144

–

52.

24. Moss JL, Crosnoe LE, Kim ED. Effect of rejuvenation hormones on

spermatogenesis. Fertil Steril. 2013;99:1814

–

20.

25. Mitchell RT, Mungall W, McKinnell C, Sharpe RM, Cruickshanks L,

Milne L et al. Anogenital distance (AGD) plasticity in adulthood:

Implications for its use as a biomarker of fetal androgen action.

Endocrinology 2014:en20141534.

484

J Assist Reprod Genet (2015) 32:479

–

484